Silver nanoparticles loaded graphene-poly-vinylpyrrolidone composites as an effective recyclable antimicrobial agent

Suguna Perumal, Raji Atchudan, Srinivasan Ramalingam, S. Aldawood, Natarajan Devarajan, Wonmok Lee, Yong Rok Lee

Research output: Contribution to journalArticlepeer-review

Abstract

Silver nanoparticles (AgNPs) are often used as antibacterial agents. Here, graphene-silver nanoparticles (G-Ag) and graphene-silver nanoparticles poly-vinylpyrrolidone (G-AgPVPy) were prepared by chemical reduction and in-situ polymerization of vinylpyrrolidone (VPy). The prepared G-Ag and G-AgPVPy composites were characterized using various techniques. The size of the AgNPs on the graphene surface in the prepared G-Ag and G-AgPVPy composites was measured as ∼20 nm. The graphene sheets size in the G-Ag and G-AgPVPy composites were measured as 6.0–2.0 μm and 4.0–0.10 μm, respectively, which are much smaller than graphene sheets in graphite powder (GP) (10.0–3.0 μm). The physicochemical analysis confirmed the formation of G-Ag and G-AgPVPy composites and even the distribution of AgNPs and PVPy on the graphene sheets. The synthesized composites (G-AgPVPy, G-Ag) exhibited a broad-spectrum antibacterial potential against both Gram-negative and Gram-positive bacteria. The lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were calculated as >40 μg/mL using G-Ag and GP, while G-AgPVPy showed as 10 μg/mL against Staphylococcus aureus. Among GP, G-Ag, and G-AgPVPy, G-AgPVPy disturbs the cell permeability, damages the cell walls, and causes cell death efficiently. Also, G-AgPVPy was delivered as a significant reusable antibacterial potential candidate. The MIC value (10 μg/mL) did not change up to six subsequent MIC analysis cycles.

Original languageEnglish
Article number114706
JournalEnvironmental Research
Volume216
DOIs
StatePublished - 1 Jan 2023

Keywords

  • Bacillus cereus
  • Escherichia coli
  • Graphite powder
  • Poly-vinylpyrrolidone
  • Salmonella typhimurium
  • Staphylococcus aureus

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