TY - CHAP
T1 - Isolate new microalgal strain for biodiesel production and using FTIR spectroscopy for assessment of pollutant removal from Palm Oil Mill Effluent (POME)
AU - Kamyab, Hesam
AU - Chelliapan, Shreeshivadasan
AU - Md Din, Mohd Fadhil
AU - Lee, Chew Tin
AU - Rezania, Shahabaldin
AU - Khademi, Tayebeh
AU - Bong, Cassendra Phun Chien
N1 - Funding Information:
The authors would like to acknowledge IPASA, RAZAK School, and MJIIT of Universiti Teknologi Malaysia (UTM) for providing adequate facilities to conduct this research, and Algaetech Sdn. Bhd for consultation. The first author is a researcher of Universiti Teknologi Malaysia (UTM) under the Post-Doctoral Fellowship Scheme (PDRU Grant) for the project: “Alternative Innovation of Enhancement Technologies for Algal Oil Extraction” (Vot No. Q.J130000.21A2.03E31).
Publisher Copyright:
Copyright © 2018, AIDIC Servizi S.r.l.
PY - 2018
Y1 - 2018
N2 - In tropical countries, the palm oil industry discharges a large amount of wastewater. The wastewater can serve as an economical nutrient source or substrate that can support the cultivation of microalgae. This study aimed to identify the local species of microalgae potentially existing in the industrial wastewater of palm oil mill effluent (POME). POME was selected as the key source of waste due to its higher potential in producing lipids from microalgae as biofuel substrate. A novel green microalgal strain was isolated from POME of Kahang-Johor west palm oil mill in Malaysia and was identified as Chlamydomonas sp. and subsequently named UTM 98 with Catalogue No. of KR349061. This study emphasised the effectiveness of POME as the main carbon source to maintain the growth of microalgae and simultaneously to increase the lipid content. In this study, Fourier Transform Infrared spectroscopy (FTIR) and Gas Chromatography (GC-FID) were used to identify and quantify lipids in the freshwater microalgae. Cultivation of microalgae were initially carried out in 250 mL Erlenmeyer flask containing 100 mL medium at ± 30 °C with continuous illumination (± 14 μmol-1 m-2 s-1) and up to 20 d of cultivations. Results demonstrated that on the chromatogram, the highest retention achieved is belong to palmitic acid (C16:0). Chlamydomonas incerta (C. incerta) species is found to contain shorter chain fatty acids, mainly 16 - 18 carbon length, which is ideal for biodiesel production. FTIR spectrum of POME treated biomass displayed the shifting of peak at 591 cm−1 and also removal of C-Cl stretching. The spectrum of POME effluent treated biomass revealed broad peak at 3,430 cm−1. The results of SEM micrographs showed that, after treating POME with C. incerta, the cells became slightly rough and corrugated textures and some particles were found on the surface of the cell wall. Using POME as a rich carbon and nutrient source is also a promising approach either as natural environment treatment or as high-lipid-content raw material for production of biofuel.
AB - In tropical countries, the palm oil industry discharges a large amount of wastewater. The wastewater can serve as an economical nutrient source or substrate that can support the cultivation of microalgae. This study aimed to identify the local species of microalgae potentially existing in the industrial wastewater of palm oil mill effluent (POME). POME was selected as the key source of waste due to its higher potential in producing lipids from microalgae as biofuel substrate. A novel green microalgal strain was isolated from POME of Kahang-Johor west palm oil mill in Malaysia and was identified as Chlamydomonas sp. and subsequently named UTM 98 with Catalogue No. of KR349061. This study emphasised the effectiveness of POME as the main carbon source to maintain the growth of microalgae and simultaneously to increase the lipid content. In this study, Fourier Transform Infrared spectroscopy (FTIR) and Gas Chromatography (GC-FID) were used to identify and quantify lipids in the freshwater microalgae. Cultivation of microalgae were initially carried out in 250 mL Erlenmeyer flask containing 100 mL medium at ± 30 °C with continuous illumination (± 14 μmol-1 m-2 s-1) and up to 20 d of cultivations. Results demonstrated that on the chromatogram, the highest retention achieved is belong to palmitic acid (C16:0). Chlamydomonas incerta (C. incerta) species is found to contain shorter chain fatty acids, mainly 16 - 18 carbon length, which is ideal for biodiesel production. FTIR spectrum of POME treated biomass displayed the shifting of peak at 591 cm−1 and also removal of C-Cl stretching. The spectrum of POME effluent treated biomass revealed broad peak at 3,430 cm−1. The results of SEM micrographs showed that, after treating POME with C. incerta, the cells became slightly rough and corrugated textures and some particles were found on the surface of the cell wall. Using POME as a rich carbon and nutrient source is also a promising approach either as natural environment treatment or as high-lipid-content raw material for production of biofuel.
UR - http://www.scopus.com/inward/record.url?scp=85047312804&partnerID=8YFLogxK
U2 - 10.3303/CET1863016
DO - 10.3303/CET1863016
M3 - Chapter
AN - SCOPUS:85047312804
VL - 63
SP - 91
EP - 96
BT - Chemical Engineering Transactions
PB - Italian Association of Chemical Engineering - AIDIC
ER -
