Development of antibody against drug-resistant respiratory syncytial virus: Rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system

Hyeran Kim, Seul Gee Hwang, Kyeonghye Guk, Yoonji Bae, Hwangseo Park, Eun Kyung Lim, Taejoon Kang, Juyeon Jung

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Respiratory syncytial virus (RSV) infections are associated with severe bronchiolitis or pneumonia. Although palivizumab is used to prevent RSV infections, the occurrence of palivizumab-resistant RSV strains is increasing, and these strains pose a threat to public health. Herein, we report an antibody with affinity to the S275F RSV antigen, enabling the specific detection of palivizumab-resistant RSV strains. Experimental and simulation results confirmed the affinity of the antibody to the S275F RSV antigen. Furthermore, we developed a rapid S275F RSV antigen detection method using a split superfolder green fluorescent protein (ssGFP) that can interact with the antibody. In the presence of the mutant virus antigen, ssGFP emitted fluorescence within 1 min, allowing the rapid identification of S275F RSV. We anticipate that the developed antibody would be useful for the precise diagnosis of antiviral drug-resistant RSV strains and help treat patients with RSV infections.

Original languageEnglish
Article number113593
JournalBiosensors and Bioelectronics
Volume194
DOIs
StatePublished - 15 Dec 2021

Keywords

  • Antibody
  • Drug-resistance
  • Green fluorescent protein
  • Respiratory syncytial virus
  • S275F mutation

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