@article{cff0ef4a9d8d446182fe006db8cca0b5,
title = "Development of antibody against drug-resistant respiratory syncytial virus: Rapid detection of mutant virus using split superfolder green fluorescent protein-antibody system",
abstract = "Respiratory syncytial virus (RSV) infections are associated with severe bronchiolitis or pneumonia. Although palivizumab is used to prevent RSV infections, the occurrence of palivizumab-resistant RSV strains is increasing, and these strains pose a threat to public health. Herein, we report an antibody with affinity to the S275F RSV antigen, enabling the specific detection of palivizumab-resistant RSV strains. Experimental and simulation results confirmed the affinity of the antibody to the S275F RSV antigen. Furthermore, we developed a rapid S275F RSV antigen detection method using a split superfolder green fluorescent protein (ssGFP) that can interact with the antibody. In the presence of the mutant virus antigen, ssGFP emitted fluorescence within 1 min, allowing the rapid identification of S275F RSV. We anticipate that the developed antibody would be useful for the precise diagnosis of antiviral drug-resistant RSV strains and help treat patients with RSV infections.",
keywords = "Antibody, Drug-resistance, Green fluorescent protein, Respiratory syncytial virus, S275F mutation",
author = "Hyeran Kim and Hwang, {Seul Gee} and Kyeonghye Guk and Yoonji Bae and Hwangseo Park and Lim, {Eun Kyung} and Taejoon Kang and Juyeon Jung",
note = "Funding Information: This research was supported by National R&D Programs through National Research Foundation (NRF) of Korea funded by Ministry of Science and ICT (MSIT) of Korea ( NRF-2020R1A2C1010453 , NRF-2021M3E5E3080844 , NRF-2019R1C1C1006867 , NRF-2021M3H4A1A02051048 , NRF-2021M3E5E3080379 , and NRF-2018M3A9E2022821 ), Global Frontier Program through Center for BioNano Health-Guard funded by MSIT of Korea ( H-GUARD_2014M3A6B2060507 and H-GUARD_2013M3A6B2078950 ), Technology Development Program for Biological Hazards Management in Indoor Air through Korea Environment Industry & Technology Institute (KEITI) funded by Ministry of Environment (ME) of Korea ( 2021003370003 ), Industrial Technology Alchemist Program funded by Ministry of Trade, Industry, and Energy (MOTIE) of Korea ( 20012435 ), Nanomedical Devices Development Program of National Nano Fab Center ( CSM2105M101 ), and KRIBB Research Initiative Program ( 1711134081 and 1711134038 ). Publisher Copyright: {\textcopyright} 2021 The Authors",
year = "2021",
month = dec,
day = "15",
doi = "10.1016/j.bios.2021.113593",
language = "English",
volume = "194",
journal = "Biosensors and Bioelectronics",
issn = "0956-5663",
publisher = "Elsevier Ltd.",
}